Abstract

Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.