Abstract
Microtubule-severing protein (MTSP) is critical for the survival of both mitotic and postmitotic cells. However, the study of MTSP during meiosis of mammalian oocytes has not been reported. We found that spastin, a member of the MTSP family, was highly expressed in oocytes and aggregated in spindle microtubules. After knocking down spastin by specific siRNA, the spindle microtubule density of meiotic oocytes decreased significantly. When the oocytes were cultured in vitro, the oocytes lacking spastin showed an obvious maturation disorder. Considering the microtubule-severing activity of spastin, we speculate that spastin on spindles may increase the number of microtubule broken ends by severing the microtubules, therefore playing a nucleating role, promoting spindle assembly and ensuring normal meiosis. In addition, we found the colocalization and interaction of collapsin response mediator protein 5 (CRMP5) and spastin in oocytes. CRMP5 can provide structural support and promote microtubule aggregation, creating transportation routes, and can interact with spastin in the microtubule activity of nerve cells (30). Knocking down CRMP5 may lead to spindle abnormalities and developmental disorders in oocytes. Overexpression of spastin may reverse the abnormal phenotype caused by the deletion of CRMP5. In summary, our data support a model in which the interaction between spastin and CRMP5 promotes the assembly of spindle microtubules in oocytes by controlling microtubule dynamics, therefore ensuring normal meiosis.
Highlights
Microtubule-severing protein (MTSP) is highly critical for the survival of both mitotic and post-mitotic cells.the study of Microtubule-severing proteins (MTSPs) in the meiosis of mammalian oocyte has not been reported
Combining with the microtubule severing activity of spastin, we speculate that spastin on spindle may increase the microtubule broken ends by severing microtubules, playing a nucleating role, promoting spindle assembly and ensuring normal meiosis
Conclusions :To sum up, our data support a model in which the interaction between spastin and collapsin response mediator protein 5 (CRMP5) promotes the assembly of spindle microtubules in oocyte by controlling microtubule dynamics, ensuring normal meiosis
Summary
Microtubule-severing protein (MTSP) is highly critical for the survival of both mitotic and post-mitotic cells.the study of MTSP in the meiosis of mammalian oocyte has not been reported. Microtubule-severing proteins (MTSPs) generate internal breaks in microtubules (MTs). They are conserved in eukaryotes from ciliates to mammals, and their function is important in diverse cellular processes ranging from cilia biogenesis to cell division, phototropism, and neurogenesis. Many studies have shown that MTSPs play an important role in mitosis through their MT severing activity. Fidgetin [16,18], fidgetin-like 1 [19], katanin-like 1 [20] and katanin-like 2 [21] are considered essential for mitosis They show different localization patterns in cells, and the absence of these proteins affects the assembly of spindle microtubules in varying degrees
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
