Spasmolytic effect of 1,8 cineole is mediated through calcium channel blockade in the bovine ileum.

Abstract

Gastrointestinal motility disorders include increased or decreased movements. Other studies have shown that herbal components, for example, essential oils can modify the increase and decrease of gastrointestinal movements of ruminants. The 1,8-cineole being obtained from the essential oil of many plants has several effects. The present study has investigated the effect of 1,8-cineole on the contractility of bovine ileum smooth muscle. The experiment was performed on the circular smooth muscle of ileum samples taken from slaughtered cows in the organ bath. Seven cumulative concentrations of 1,8-cineole from 1.00 to 1,000 µg mL-1 were added to tissue samples. The used solution was Tyrode's solution aerated with a mixture of 95.00% oxygen and 5.00% carbon dioxide, and the temperature was set at 37.00 ˚C. The effects of 1,8-cineole on baseline contractions and three induced contractions with barium chloride, potassium chloride, and carbachol were investigated. The effects of 1,8-cineole, and verapamil (standard calcium channel blocker) on calcium channels were assessed. The results revealed that 1,8-cineole significantly inhibited spontaneous contractions as well as all spasmogen-induced contractions. The 1,8-cineole exerts its myorelaxant properties by inhibiting calcium channels in smooth muscle. It seems that 1,8-cineole has a good potential for producing antispasmodics or gastrointestinal motility modulators in veterinary medicine.