Sparkling Reflective Stacks of Purine Crystals in the Nudibranch Flabellina iodinea.

Abstract

Although pigments contribute to much of the brilliant purple and orange coloration of the aeolid nudibranch Flabellina iodinea, the optical appearance of the animal was found to be augmented by dynamically sparkling, brightly reflective material in cells located throughout its epidermis. Electron microscopy revealed that specialized cells most abundant near the epithelial basal lamina contain numerous multilayer stacks of crystals, each within a fragile membrane capsule. High-resolution light microscopy of tissue sections showed that these crystalline stacks intermittently reflect light, with a temporally dynamic, sparkling appearance, suggesting that they are free to move-a phenomenon also observed in the live, intact whole animal and in the purified crystal stacks as well. Thin-layer chromatography and ultraviolet spectrometry show that the crystals isolated from all epithelial tissues are identical in composition, with guanine being the major component and its derivative, hypoxanthine, a minor component, regardless of the tissue's pigmentary color. Electron diffraction of the crystals purified separately from the orange and purple tissues exhibits nearly identical lattice parameters that closely match those measured for guanine crystals, which are widely distributed in other biophotonic systems ranging from marine invertebrates to terrestrial vertebrates. Heterogeneity of the thickness and spacing of the crystals within their stacks accounts for their broadband silvery reflectance. The optical appearance of the epidermis of this nudibranch thus results from the interaction of incident light with mobile stacks of purine crystals, augmenting the effects of its pigmentary colors.