Séparation électrophorétique des isoenzymes de la phosphatase alcaline par le Kit Hydragel 15 Iso-PAL®

Abstract

We evaluated a new electrophoresis Kit (Hydragel 15 Iso-PAL®, Sebia) for the separation of alkaline phosphatase (ALP) isoforms. This agarose gel technique uses wheat germ lectin affinity for sialic acids present on ALP isoenzymes. Similar results were obtained between serum and plasma (lithium heparinate), particularly for the bone fraction (< 5% difference). In healthy subjects, bone and liver H1 fractions are the two major components of ALP activity (up to 90%) with a ratio close to 1/1. This technique displays a practical advantage for laboratories, due to the absence of sample pre-treatment, the use of a single gel and automation on the Hydrasys® system (2 h analysis time for 7 patients). This method also offers a clinical advantage for bone and hepatobiliary diseases, allowing a whole panorama of ALP activity.