Abstract
Separation of isozymes hydrolysing LNA has been obtained by electrophoresis in various media. Vertical starch gel electrophoresis had previously allowed the isolation of three isozymes in sera of normal pregnant women in the last third of pregnancy. Vertical electrophoresis in acrylamide gel was adapted in order to obtain a separation similar to that in starch gel. A discontinuous system consisting mainly of 8.75% acrylamide gel in 0.052 M borate buffer (pH 8.6) between electrode vessels containing 0.3 M borate-0.06 M NaOH (pH 8.6) is described. Application of 5 mA current (tension 105 to 125 V) during 60 min on 10-μl samples of human serum resulted in a good separation of three isozymes hydrolysing LNA. This new analytical application confirms the high degree of reproducibility, sensitivity and speed of acrylamide electrophoresis.
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