Abstract
Measurement of chlorophyll content using destructive methods is not efficient due to a large number of samples, cost, and time needed. Estimationof chlorophyll content by nondestructive methods using handheld chlorophyll meter may be considered to improve efficiency. This research aimed to determine the formula to convert SPAD-502 and atLEAF CHL PLUS values (relative indicator of chlorophyll content) to estimated (absolute) rubber leaves chlorophyll content. Twenty leaves of rubber plant were measured using SPAD-502 and atLEAF CHL PLUS at the same time to determine SPAD-502 and atLEAF CHL PLUS values. The measured leaves were then collected to determine the chlorophyll content using a standard laboratory procedure. Regression and correlation analyses (among 3 methods) were conducted using SAS v.9 software. The results showed that between SPAD-502 and atLEAF CHL PLUS values were closely correlated, hence both of the devices can substitute each other to estimate rubber leaf chlorophyll content. In addition, the relationship between atLEAF CHL PLUS and SPAD-502 values with actual chlorophyll content of rubber clone SP 217, PB 260, GT1, and all clones (general) were significant with high coefficient of determination (R2) as well as low Root Mean Square Error (RMSE) and Coefficient of Variation (CV). Therefore, by using formula determined in this study, both atLEAF CHL PLUS and SPAD-502 can be suggested for accurate, fast, and non-destructive estimation of chlorophyll content of rubber plant leaf.
Highlights
Chlorophyll is specialized light-absorbing green pigment that play an essential role in capturing light energy for photosynthesis processes (Limantara et al, 2015; Taiz & Zeiger, 2002)
The results showed that between SPAD-502 and atLEAF CHL PLUS values were closely correlated, both of the devices can substitute each other to estimate rubber leaf chlorophyll content
Both SPAD-502 and atLEAF CHL PLUS did not produce an absolute estimation of chlorophyll content data, whether it is chlorophyll a, b, or total chlorophyll
Summary
Chlorophyll is specialized light-absorbing green pigment that play an essential role in capturing light energy for photosynthesis processes (Limantara et al, 2015; Taiz & Zeiger, 2002). There are some handheld chlorophyll meter commonly used to measure chlorophyll content of higher plant leaf based on the light absorbance and or reflectance by intact leaves (Richardson et al, 2002), for example SPAD-502 chlorophyll meter (Minolta Camera Co., Ltd., Japan) and AtLEAF CHL PLUS chlorophyll meter (FT Green LLC, Willmington, DE). These devices provide simple, quick, and inexpensive measurement of chlorophyll content (Coste et al, 2010; Ruiz-Espinoza et al, 2010; Zhu et al, 2012). Measurement of chlorophyll content based on absorbance and or reflectance of light by leaf generally generates an index value not an absolute chlorophyll content per leaf area unit or per leaf tissue weight (Ruiz-Espinoza et al, 2010)
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