Space-Efficient Indexing of Spaced Seeds for Accurate Overlap Computation of Raw Optical Mapping Data.

Abstract

A key problem in processing raw optical mapping data (Rmaps) is finding Rmaps originating from the same genomic region. These sets of related Rmaps can be used to correct errors in Rmap data, and to find overlaps between Rmaps to assemble consensus optical maps. Previous Rmap overlap aligners are computationally very expensive and do not scale to large eukaryotic data sets. We present Selkie, an Rmap overlap aligner based on a spaced (l,k)-mer index which was pioneered in the Rmap error correction tool Elmeri. Here we present a space efficient version of the index which is twice as fast as prior art while using just a quarter of the memory on a human data set. Moreover, our index can be used for filtering candidates for Rmap overlap computation, whereas Elmeri used the index only for error correction of Rmaps. By combining our filtering of Rmaps with the exhaustive, but highly accurate, algorithm of Valouev et al. (2006), Selkie maintains or increases the accuracy of finding overlapping Rmaps on a bacterial dataset while being at least four times faster. Furthermore, for finding overlaps in a human dataset, Selkie is up to two orders of magnitude faster than previous methods.