Abstract
Inflammatory bowel disease have been linked to several health issues, including high risk of low bone mineral density. Danio rerio (zebrafish) is a good model to verify the effects of intestinal inflammation, since its gastrointestinal and immune systems are closely related to that of mammalians. Zebrafish is also a powerful model to study bone metabolism using the scale as the read-out model. Food strongly impacts zebrafish gut physiology, and it is well known that soybean meal induces intestinal inflammation. Adult zebrafish fed with defatted soybean meal (SBM) exhibited an intestinal inflammation evidenced by morphological alterations, inflammatory infiltrate, and increased mRNA expression of inflammatory cytokines (IL-1β, IL-6, IL-8, IL-10, TGFβ, TNF-α). The peak of acute intestinal inflammation, spanning between week 2 and 3, correlates with a transitory osteoporosis-like phenotype in the scale border. Later, a chronic inflammatory condition, associated with persistent IL-8 expression, correlates with the progression of resorption lacunae in the scale center. Both types of resorption lacunae were associated with intense osteoclastic tartrate-resistant acid phosphatase (TRAP) activity. After 3 weeks of SBM treatment, osteoclast activity decreased in the scale border but not in the center. At the same time, alkaline phosphatase (ALP) is activated in the border to repair the bone matrix. This model can contribute to elucidate in vivo the molecular mechanisms that links intestinal inflammation and bone metabolism in IBD.
Highlights
Inflammatory bowel disease (IBD) was linked to several health issues in human patients, including high risk of low bone mineral density and increased risk of fractures [1].Danio rerio is an excellent model to verify the various adverse effects of intestinal inflammation, including any effects on bone tissue
The aim of this study was to analyze bone tissue alterations in an adult zebrafish model due to experimental IBD induced by soybean meal (SBM) treatment
Meal Induces in Adult zebrafish were Intestinal fed for 5 Inflammation weeks with 50%
Summary
Danio rerio (zebrafish) is an excellent model to verify the various adverse effects of intestinal inflammation, including any effects on bone tissue. Zebrafish is a powerful model to study bone tissue in the adult organism using the scale as read-out model due to its high accessibility, number of samples, bone tissue similarity, and transparency [2]. It must be considered that the zebrafish immune system is highly related to that of mammals in both the innate and adaptive branches [3]. The zebrafish intestinal tract consists of a twicefolded long tube located in the abdominal cavity and is arbitrarily divided based on morphological characteristics in the intestinal bulb, the mid and the posterior intestinal segments. Zebrafish intestine has comparable absorptive and secretory functions with those of mammals and is itself organized in concentric layers, even if supporting connective tissue
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