Abstract
Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybeans (Glycine max). In this study, an interaction between the SMV P3 protein and cytochrome b5 was detected by yeast two-hybrid assay, and bimolecular fluorescence complementation assay showed that the interaction took place at the cell periphery. Further, the interaction was confirmed by co-immunoprecipitation analysis. Quantitative real-time polymerase chain reaction analysis revealed that GmCYB5 gene was differentially expressed in resistant and susceptible soybean plants after inoculation with SMV-SC15 strain. To test the involvement of this gene in SMV resistance, the GmCYB5 was silenced using a bean pod mottle virus (BPMV)-based vector construct. Results showed that GmCYB5-1 was 83% and 99% downregulated in susceptible (NN1138-2) and resistant (RN-9) cultivars, respectively, compared to the empty vector-treated plants. Silencing of GmCYB5 gene promotes SMV replication in soybean plants. Our results suggest that during SMV infection, the host CYB5 protein targets P3 protein to inhibit its proliferation. Taken together, these results suggest that CYB5 is an important factor in SMV infection and replication in soybeans, which could help soybean breeders develop SMV resistant soybean cultivars.
Highlights
Soybean (Glycine max (L.) Merr.) is an important protein and oil crop
We conducted a yeast two-hybrid (Y2H) screen to identify soybean proteins that interacted with the Soybean mosaic virus (SMV) P3 protein
A widely used method to characterize the function of known proteins is to identify their their interacting factors and infer their roles based on the functions of known interacting factors
Summary
Soybean mosaic virus (SMV), a member of Potyvirus, is seed-borne and aphid-transmitted. SMV causes severe reduction of soybean yield and destroys the seed quality [1,2]. In the United States, 98 isolates of SMV were divided into seven strains, namely G1 to G7, according to differential phenotypic reactions to five resistant and two susceptible varieties of soybeans [3,4]. Resistance loci containing dominant resistance (R) genes named. Based on the reaction to specific soybean cultivars, the SMV isolates were classified into 21 strains in China and were named SC1 to SC21 [16,17,18]. Resistance derived from the resistant to SC (Rsc) loci to the strains from China have been mapped to chromosome
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
