Abstract
Soyasapogenol is a soyasaponin aglycone, which has been suggested to exert a more potent function than the glycoside form. In this study, the effect of soyasapogenol A and B on cultured adipocyte cell function was investigated using mouse 3T3-L1 adipocyte cells. 3T3-L1 cells were treated with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine for differentiation to adipocytes, and the cells were then cultured in the presence of soyasapogenol A or B (6.25 or 12.5 µM). The media were harvested and refreshed every 2 d. After a 10 d culture, the cells were harvested and the triglyceride content of the cells was determined. The triglyceride content of soyasapogenol B-treated cells was significantly lower than those of vehicle-treated cells. Glycerol and free fatty acid levels in the soyasapogenol-treated cell media were higher than those in vehicle cells. However, there was no difference in the level of adipose triglyceride lipase among soyasapogenol A-, soyasapogenol B-, and vehicle-treated cells. The secreted adiponectin and resistin levels of soyasapogenol-treated cell media were also different compared with those of vehicle-treated cells. Especially, the secreted resistin level in soyasapogenol B-treated cell media was obviously reduced compared with that of vehicle-treated cells. Taken together, these results suggest that soyasapogenol B exerted an anti-obesity and anti-diabetic effect on adipocytes by lowering the cellular triglyceride level by accelerating triglyceride lipolysis with reduced resistin secretion.
Highlights
Metabolic syndrome (MS) is a group of metabolic factors that are associated with cardiovascular disease and type 2 diabetes [1,2]
There was no significant difference in triglyceride level between cells treated with soyasapogenol A or soyasapogenol B at either concentration. 3T3-L1 cell viability was measured using a cell proliferation assay kit
These results suggest that soyasapogenol A and B enhanced lipolysis in adipocyte cells and accompanied the reduction of triglycerides in lipid droplets stained by oilred O and that the potency of accelerating lipolysis was higher with soyasapogenol B than it was with soyasapogenol A
Summary
Metabolic syndrome (MS) is a group of metabolic factors that are associated with cardiovascular disease and type 2 diabetes [1,2]. Obesity is linked with the induction of insulin resistance in adipose tissue, which results in several pathologies related to type 2 diabetes. Soybeans have been reported to promote various health functions such as lipid metabolism [6] They include many functional food components, such as soy protein, β-conglycinin, isoflavones, soy peptides [6], lectin, trypsin inhibitor, lecithin, tocopherol, and saponins. Their functional components have been reported to lower cholesterol and triglycerides and to improve lipid metabolism [7,8,9], the exact mechanism of action exerted by those functional components has not been fully elucidated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
