Abstract
BackgroundIn preimplantation mammalian development the transcription factor Sox2 (SRY-related HMG-box gene 2) forms a complex with Oct4 and functions in maintenance of self-renewal of the pluripotent inner cell mass (ICM). Previously it was shown that Sox2−/− embryos die soon after implantation. However, maternal Sox2 transcripts may mask an earlier phenotype. We investigated whether Sox2 is involved in controlling cell fate decisions at an earlier stage.Methods and FindingsWe addressed the question of an earlier role for Sox2 using RNAi, which removes both maternal and embryonic Sox2 mRNA present during the preimplantation period. By depleting both maternal and embryonic Sox2 mRNA at the 2-cell stage and monitoring embryo development in vitro we show that, in the absence of Sox2, embryos arrest at the morula stage and fail to form trophectoderm (TE) or cavitate. Following knock-down of Sox2 via three different short interfering RNA (siRNA) constructs in 2-cell stage mouse embryos, we have shown that the majority of embryos (76%) arrest at the morula stage or slightly earlier and only 18.7–21% form blastocysts compared to 76.2–83% in control groups. In Sox2 siRNA-treated embryos expression of pluripotency associated markers Oct4 and Nanog remained unaffected, whereas TE associated markers Tead4, Yap, Cdx2, Eomes, Fgfr2, as well as Fgf4, were downregulated in the absence of Sox2. Apoptosis was also increased in Sox2 knock-down embryos. Rescue experiments using cell-permeant Sox2 protein resulted in increased blastocyst formation from 18.7% to 62.6% and restoration of Sox2, Oct4, Cdx2 and Yap protein levels in the rescued Sox2-siRNA blastocysts.Conclusion and SignificanceWe conclude that the first essential function of Sox2 in the preimplantation mouse embryo is to facilitate establishment of the trophectoderm lineage. Our findings provide a novel insight into the first differentiation event within the preimplantation embryo, namely the segregation of the ICM and TE lineages.
Highlights
Sox genes are expressed throughout embryogenesis and encode a subclass of high mobility group (HMG) box proteins driving cell fate decisions by acting as transcription factors and architectural components of chromatin [1,2]
We conclude that the first essential function of Sox2 in the preimplantation mouse embryo is to facilitate establishment of the trophectoderm lineage
Our findings provide a novel insight into the first differentiation event within the preimplantation embryo, namely the segregation of the inner cell mass (ICM) and TE lineages
Summary
Sox genes are expressed throughout embryogenesis and encode a subclass of high mobility group (HMG) box proteins driving cell fate decisions by acting as transcription factors and architectural components of chromatin [1,2]. Expression of Sox is observed in mouse and human eye lens [6]; in humans, heterozygous loss-of-SOX2 function causes several defects including bilateral anophthalmia [7], and defects in the hypothalamo-pituitarygonadal axis [8]. It is essential for inner ear sensory organ [9] and taste bud sensory cell [10] development. In preimplantation mammalian development the transcription factor Sox (SRY-related HMG-box gene 2) forms a complex with Oct and functions in maintenance of self-renewal of the pluripotent inner cell mass (ICM). We investigated whether Sox is involved in controlling cell fate decisions at an earlier stage
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