Sox19 regulates ovarian steroidogenesis in common carp

Abstract

In teleost, ovarian steroidogenesis governed by the neuroendocrine system is also regulated by several transcription factors of gonadal origin. Investigating the synchronized interactions between the transcriptional and the hormonal factors is vital to comprehend the mechanisms that lead to gonadal differentiation. This study signifies the role of sry-related box (sox) 19 in ovarian steroidogenesis regulation of the common carp, Cyprinus carpio. Analysis of tissue distribution displayed higher sox19 expression in brain and ovary, and gonadal ontogeny showed higher expression of sox19 at 80 days post hatch (dph). Higher sox19 mRNA expression during spawning and increase of sox19 post human chorionic gonadotropin induction substantiate gonadotropin dependency. Estradiol-17β treatment but not 17α–methyl-di-hydroxy-testosterone to 50 dph common carp for inducing mono-sex, elevated sox19 expression substantially. Sox19 protein was observed in granulosa cells of the follicular layer in common carp ovary. Higher sox19 expression was detected in isolated granulosa and theca cells, in vitro. Transient gene silencing with sox19-siRNA caused downregulation of various ovary-related genes including those specific to activator protein-1 factors, fibroblast growth factors, wnt-signaling, steroidogenic genes along with certain transcription factors. Serum 17α, 20β-dihydroxy-4-pregnen-3-one and estradiol-17β reduced significantly post sox19 silencing, in vivo. Concomitantly, a decrease in aromatase activity was detected post sox19-siRNA treatment, in vivo. This study demonstrates the impact of sox19 in the regulation of common carp ovarian growth and steroidogenesis.