Abstract
The study aimed to investigate the effects of Sry-like high mobility group box 15 (SOX15) on proliferation and migration of endometrial cancer (EC) cells. Immunohistochemistry (IHC) was applied to determine the expression of SOX15 in EC tissues and adjacent tissues. We used cell transfection method to construct the HEC-1-A and Ishikawa cell lines with stable overexpression and low expression SOX15. Reverse-transcription quantitative real-time PCR (RT-qPCR) and Western blot were performed to examine expression of SOX15 mRNA and SOX15 protein, respectively. By conducting a series of cell proliferation assay and migration assay, we analyzed the influence of SOX15 overexpression or low expression on EC cell proliferation and migration. The expression of SOX15 mRNA and protein in EC tissues was significantly lower than that in adjacent tissues. After lentivirus-transfecting SOX15, the expression level of SOX15 mRNA and protein was significantly increased in cells of SOX15 group, and decreased in sh-SOX15 group. Overexpression of SOX15 could suppress cell proliferation, while down-regulation of SOX15 increased cell proliferation. Flow cytometry results indicated that overexpression of SOX15 induced the ratio of cell-cycle arrest in G1 stage. In addition, Transwell migration assay results showed that SOX15 overexpression significantly inhibited cell migration, and also down-regulation of SOX15 promoted the migration. As a whole, SOX15 could regulate the proliferation and migration of EC cells and up- regulation of SOX15 could be valuable for EC treatment.
Highlights
Endometrial cancer (EC) is recognized as the fourth most widespread gynecologic malignancy in the United States, with an estimated 60000 new cases and 10500 deaths in 2016 [1]
According to reverse-transcription quantitative real-time PCR (RT-qPCR) results, both in HEC-1-A and Ishikawa cells, the expression of Sry-like high mobility group box 15 (SOX15) mRNA in SOX15 group was conspicuously higher than the control group (P
The expression of SOX15 mRNA in the sh-SOX15 group was lower than sh-NC group (P
Summary
Endometrial cancer (EC) is recognized as the fourth most widespread gynecologic malignancy in the United States, with an estimated 60000 new cases and 10500 deaths in 2016 [1]. It is the sixth most common cancer amongst women worldwide, with nearly 319600 cases diagnosed in a year [2]. More aggressive histological variants are Type II neoplasms, occupying approximately 10% of total EC cases [4]. Total hysterectomy with bilateral salpingo-oophorectomy is often considered an effective and primary treatment for EC patients, as nearly 75% of women with stage I disease are likely to be cured by surgery alone [4]. With the development of technology, the EC studies at molecular level have been emerging [13,14,15,16]
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