Abstract
Breastfeeding is recommended for the first year of life. However, breastfeeding rates in the U.S. decline from 77% at birth to 43% by 6 months and only 13% of these infants are exclusively breastfed, while the remainder are supplemented with formula (combined feeding; CF). Herein, we developed a novel piglet model to investigate the effect of CF on the colonic transcriptome. Newborn piglets were randomized into 3 groups: sow‐reared (SR), formula fed (FF) and CF (n=6 per group). SR remained with the sow 24h/day and FF were fed a sow milk replacer. CF piglets were sow‐reared for 5d and were then rotated between the sow and formula feeding every 12h. On d21, ascending colon was collected and mRNA was extracted. Gene expression was assessed using a porcine microarray (Agilent). Among 25,273 probes, 449 were differentially expressed (FDR p<0.2). Bioinformatic analyses using MetaCore, revealed that the most significant GeneGo pathway maps were oxidative phosphorylation, apoptosis, immune response, lipid metabolism and cholesterol biosynthesis. On‐going analyses are investigating relationships between host gene expression and composition of the colonic microbiota. (BMS Freedom to Discover Award)
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