Abstract
Microcyclus ulei (P. Henn.) Arx was isolated by depositing fresh conidia on plain agar and then transferring it to potato sucrose agar. Dried rubber leaf extract stimulated growth, but some brands of agar inhibited growth and sporulation. Two morphological strains occurred amongst field isolations. The free sporulating strain appeared to be associated with resistant clones. One isolate produced ascospores. Three distinct colony types were formed from cultures of laboratory-produced conidia and variants with a high degree of sporulation were obtained by successive subcultures. Single conidium cultures differed in mycelial growth, conidial and pycnidiospore production but single ascospore cultures were uniform in appearance. The fungus grew best at 23 °C, produced most conidia after three weeks, but optimum germination occurred with younger spores. Stromata were produced saprophytically on leaf disks bearing conidial lesions or pycnidia.
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