Abstract
Common bean (Phaseolus vulgaris L.) production worldwide is hampered by Fusarium root rot (FRR), which is caused by Fusarium solani. Screening for FRR resistance on a large scale is notoriously difficult and often yields inconsistent results due to variability within the environment and pathogen biology. A greenhouse screening assay was developed incorporating multiple isolates of F. solani to improve assay reproducibility. The Andean (ADP; n = 270) and Middle American (MDP; n = 280) Diversity Panels were screened in the greenhouse to identify genetic factors associated with FRR resistance. Forty-seven MDP and 34 ADP lines from multiple market classes were identified as resistant to FRR. Greenhouse phenotyping repeatability was confirmed via five control lines. Genome-wide association mapping using ∼200k SNPs was performed on standard phenotyping score 1–9, as well as binary and polynomial transformation of score data. Sixteen and seven significant genomic regions were identified for ADP and MDP, respectively, using all three classes of phenotypic data. Most candidate genes were associated with plant immune/defense mechanisms. For the ADP population, ortholog of glucan synthase-like enzyme, senescence-associated genes, and NAC domain protein, associated with peak genomic region Pv08:0.04–0.18 Mbp, were the most significant candidate genes. For the MDP population, the peak SNPs Pv07:15.29 Mbp and Pv01:51 Mbp mapped within gene models associated with ethylene response factor 1 and MAC/Perforin domain-containing gene respectively. The research provides a basis for bean improvement through the use of resistant genotypes and genomic regions for more durable root rot resistance.
Highlights
IntroductionCommon Bean Root Rot Resistance phylogenetically distinct species divided into 10 formae speciales
The inclusion of three of these control lines is recommended for continuing Fusarium root rot (FRR) evaluations and ongoing comparisons across research studies
VAX3 and Montcalm have been previously documented as appropriate resistant and susceptible controls, respectively (Bilgi et al, 2008; VasquezGuzman, 2016). Those observations were consistent with our results; we recommend the inclusion of these two lines in future FRR evaluations
Summary
Common Bean Root Rot Resistance phylogenetically distinct species divided into 10 formae speciales Classification method, and grouped together into three FSSC clades (O’Donnell, 2000; O’Donnell et al, 2008). Among the multiple species that cause root rot on common bean, F. solani has been documented as the most damaging root rot pathogen (Coleman, 2016). Symptoms of FRR on common bean manifest as dark brown to rust colored sunken lesions where lateral roots begin to rot (Abawi, 1989). Lesions on the lower hypocotyl coalesce as the disease progresses and results in complete rot of the root system (Abawi, 1989). FRR can cause up to 84% yield loss (Schneider et al, 2001)
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