Abstract
Accessions of Lycopersicon chilense, L. peruvianum, L.hirsutum and sixteen L. esculentum genotypes were evaluated undergreenhouse conditions for resistance to a whitefly-transmitted geminivirusisolate from Brasilia-DF (DF1). Artificial cage inoculation oftomato plants at the two true-leaf stage, using 20 viruliferous whiteflies perplant in individual insect-proof cages, ensured 100% infection ofsusceptible tomato plants. Virus infection was confirmed by symptomdevelopment and dot-blot or squash-blot hybridization. In advanced testing,accessions of L. chilense (LA 1967), L. peruvianum (CNPH784) and L. hirsutum (PI-127827) and three selected inbred lines(TY 197, TY 198 and Tx 468-1) showed no symptoms and viral DNAwas barely detectable four weeks after inoculation, indicating good sourcesof resistance to the virus.
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