Abstract
When laying odour trails, foragers of the black cocktail ant, Crematogaster peringueyi Emery, do not touch the tip of the gaster to the substrate, as do other trail-laying Forinicidae. To determine the anatomical source of the trail pheromone of this species, solvent extracts were made of various parts of the body, partially purified on silicic acid chromatographic columns, and assayed by means of an artificial trail technique. The source of the pheromone was shown to be the distal segments of the metathoracic legs and this was confirmed by observations on trail-laying behaviour. Ants place their hind legs close together and drum vigorously on the substrate with the tips of the tarsi.
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