Sorvall超遠沈装置(Density Gradient Ultracentifugation法)を用いた脂質代謝の臨床的研究(第一報)

Abstract

In order to elucidate the significance of HDL-cholesterol on the severity of coronary atherosclerosis, serum lipoprotein analysis was made in 70 patients who underwent coronary cineangio-graphy and 23 healthy volunteers.Serum lipoproteins were separated into 3 fractions of HDL, LDL and VLDL using the method of Density Gradient Ultra-centrifugation (SOR-VALL OTD-65 Ultracentrifuge and SORVALL TV-865B vertical rotor). Centrifugation was performed at 65, 000rev/min for 1hr. and 45min. (10°C).To evaluate the reliability of this method, data were compared with those methods such as the precipitating and Agarose gel electrophoresis.Following results were obtained;1) The correlation coefficient between HDL-cholesterol level measured by Ultracentrifugation and that of precipitating method was r=0.9189 (p<0.001), and also the correlation coefficient between the percentages of HDL-cholesterol in this method and in Agarose gel electrophoresis was r=0.8996 (p<0.001).2) Both Atherogenic Index (HDL-cholesterol/LDL-cholester) ol) and HDL-cholesterol level of the younger volunteers was significantly higher than those of the older ones (p<0.05).3) Both Atherogenic Index and HDL-cholesterol level of the volunteers was significantly higher than those of patients with 2 or more diseased coronary vessels (p<0.05).4) The correlation coefficient between serum HDL-cholesterol level and the number of diseased vessels was r=-0.5207 (p<0.001), and the more the number of diseased vessls increases, the fewer serum HDL-cholesterol level becomes.From these results, it was shown that the Density Gradient Ultracentrifugation was easy and useful method for serum lipoprotein analysis. And it was suggested that the severity of coronary atherosclerosis was estimated more precisely by Atherogenic Index from this method.