Abstract
ABSTRACT The objectives of this study were to evaluate goat sperm sorting in continuous Percoll® density gradients and gamete freezability, in the presence or absence of phenolic antioxidants. For this, semen pools were sorted, frozen, and evaluated. The non-selected group (NSg) presented lower progressive motility (PM), linearity (LIN), straightness (STR), and wobble (WOB) than the selected groups, and straight line velocity (VSL) compared to those with catechin or resveratrol. The amplitude of lateral head displacement (ALH) was higher in NSg, and quercetin reduced the mitochondrial membrane potential (MMP). After thawing, the NSg presented lower PM than the selected groups, VSL and VAP (average path velocity) than the selected group with or without catechin, LIN and WOB than the selected with or without catechin or resveratrol, and STR than the selected with catechin. Moreover, NSg presented higher ALH and BCF than the samples selected with or without catechin. Plasma membrane integrity and intact and living cells were higher in the selected groups, and MMP was lower in the NSg and the selected group with quercetin. Thus, centrifugation in Percoll® continuous density gradients is a viable methodology to select goat sperm compatible with the freezing, especially in the presence of catechin or resveratrol.
Highlights
The success of assisted reproduction techniques depends on methods that enable selection of sperm cells with high quality and fertilization capacity (Olivares et al, 2017)
Based on the high antioxidant power of phenolic compounds (Sarlós et al, 2002; Simos et al, 2012), the aim of this study was to evaluate the effect on gamete quality of goat sperm sorting by centrifugation in continuous Percoll® density gradients and subsequent sperm freezing, with or without added (+)-catechin, (-)-epigallocatechin gallate (EGCG), quercetin, or resveratrol
Regarding the sperm kinematics of fresh semen, the goat spermatozoa selected in Percoll® continuous density gradients showed higher (P< 0.05) progressive motility, LIN, STR, and WOB than non-selected spermatozoa, independent of the antioxidant use
Summary
The success of assisted reproduction techniques depends on methods that enable selection of sperm cells with high quality and fertilization capacity (Olivares et al, 2017). The use of sexed semen, in combination with other biotechnologies for reproduction, is considered a means to increase reproductive and productive efficiency. The large difference in the DNA content of goat spermatozoa, carrying an X versus a Y chromosome (4.4%), makes the sexing technique attractive for use in this species (Parrilla et al, 2004). The quality and fertility of the sexed and frozen semen after artificial insemination was low (Bathgate et al, 2013). Alternative methods are required that are simpler, cheaper (Promthep et al, 2016), less harmful to sperm (Evans et al, 2004), and allow the gametes to be frozen without reducing fertility (Hossepian de Lima et al, 2011)
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