Sorption of ruminal fluid derived phytase onto modified nanoparticles and determination of its desorption capacity

Abstract

Ruminal fluid of slaughtered cows was used as a free phytase source. Magnetic nano-particles (MNPs) were grafted with allyl 2,3-epoxypropyl ether, subsequent by coupling with cibacron blue. Cibacron blue as the main monomer is a known ligand for enzyme sorption. Characteristics of the coated MNPs were proved using Fourier transform infrared spectroscopy methods, transmission electron microscopy, and thermogravimetric analysis. These results were then compared with those of non-modified MNPs. Phytase which derived from clarified rumen fluid was then adsorbed at different pH, shaking time, and various levels of temperature via ionic bound. The best adsorption efficiency was recorded under conditions of pH 7, temperature of 6 °C, and shaking time of 60 minutes. Desorption phase was performed in two different shaking times and four different concentrations of NaCl solution. The highest percentage of desorption was lower than 20%, which achieved at 1 M NaCl solution in 90 minutes of shaking (P < 0.05).