Abstract
Sorption conditions (on hyper-cross-linked polystyrene) have been developed for six phenylcarboxylic acids (PCA), which are biomarkers of sepsis, from aqueous standard test solutions and conditions of elution of these acids for subsequent derivatization and analysis by gas chromatography-mass spectrometry (GC-MS). The conditions facilitate detection of PCA in solution at the level (and below) of content of these compounds in blood serum of normal patients. Group extraction of different in polarity required acids from aqueous solutions has been performed selectively with regard to cholesterol adopted as peculiar marker of high boiling interfering compounds. Application of the developed experimental procedure will make it possible to increase significantly the rate of express analysis for diagnostics of sepsis at early stages of development.
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