Abstract
BackgroundMedicinal plants harboring endophytic fungi could carry significant potential for producing bioactive secondary metabolites. Endophytic fungi serve as alternate source of interesting compounds in their natural and modified synthetic forms to treat different diseases. In this regard, endophytic microflora associated with alkaloid-rich medicinal plants Rhazya stricta is least known.ResultsWe isolated one new bioactive compound sorokiniol (1) along with two known cyclic peptides BZR-cotoxin I (2) and BZR-cotoxin IV (3) from fungal endophyte Bipolaris sorokiniana LK12. The structures of the isolated new and known compounds were elucidated through spectroscopic data, including 1D and 2D NMR (1H, 13C, HSQC, HMBC, and NOESY), mass, and UV. The known peptides (2–3) were characterized by ESI-MS, MS/MS, and by comparing the NMR data with the literature. The isolated metabolites were assayed for their role against enzyme inhibition. Compound 1 was significantly inhibitory towards acetyl cholinestrase while the other compounds (2–3) had moderate anti-lipid peroxidation and urease activities.ConclusionThe present results suggest that the endophytic microorganism associated with indigenously important medicinal plants can offer a rich source of biologically active chemical constituents which could help in discovering enzyme inhibitory lead drugs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0722-7) contains supplementary material, which is available to authorized users.
Highlights
Medicinal plants harboring endophytic fungi could carry significant potential for producing bioactive secondary metabolites
The results revealed that a new secondary metabolite, sorokiniol (1), along with two known cyclic depsipeptides, BZRcotoxin I (2) and BZR-cotoxin IV (3) (Fig. 1), have been isolated whilst the chemical structures were elaborated through advanced spectral techniques
Endophyte identification and screening bioactivity Endophytic fungus RSL-1.2 was identified by gDNA extraction, PCR sequencing and amplification of internal transcribed spacer (ITS) regions
Summary
Medicinal plants harboring endophytic fungi could carry significant potential for producing bioactive secondary metabolites. Endophytic fungi serve as alternate source of interesting compounds in their natural and modified synthetic forms to treat different diseases. In this regard, endophytic microflora associated with alkaloid-rich medicinal plants Rhazya stricta is least known. Endophytes (bacteria or fungi) reside in the internal tissues of plant at least once in their life cycle. This symbiosis can be oriented towards commensalism or mutualism between host and microbe. There have been many reports of bioactive natural products with significantly higher anticancer, insecticidal, and antimicrobial potentials from fungal endophytes [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
