Abstract
Brockman, II. E., and F. J. de Serres. (Oak Ridge Natl. Lab., Oak Ridge, Tenn.) “Sorbose toxicity” in Neurospora. Amer. Jour. Bot. 50(7): 709–714. Illus. 1963.—The effect of “sorbose toxicity” on Neurospora conidia or ascospores was compared in sorbose-fructose-glucose (S-F-G) and sorbose-sucrose (S-S) media. Many frequently encountered and difficultly controlled experimental variables may strongly affect viability in S-S media but are essentially without effect in S-F-G media. The viabilities of different mutant strains are affected to varying extents by autoclave exposure time of the S-S media but not of the S-F-G media. Ascospores are more sensitive than conidia to “sorbose kill” in S-S media. An over plating method described by New meyer (1954) for increasing ascospore “viability” is without effect when sucrose is replaced with fructose and glucose. In all experiments in which sorbose was added to induce colonial growth, “sorbose toxicity” or “sorbose kill” was eliminated or minimized by replacing sucrose with a mixture of fructose and glucose as the carbon source for Neurospora media.
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