Sonochemical synthesis of silver nanoparticles using orange juice: Cytotoxic behaviour and applications

Abstract

One-pot open beaker green synthesis of silver nano-particles (Ag NPs) by ultrasound energy at room temperature is presented. Citrus fruit-mediated Ag NPs formation was monitored by observing surface plasmon resonance (SPR) by UV–Visible measurements. Orange juice extract played a dual role as it helped in the surface capping of Ag NPs while being a reducing agent to yield phase pure silver nanoparticles of less than 10 nm diameter. The phase purity and particle diameter were confirmed by X-ray diffraction (XRD) patterns and organic functionality was established by infra-red spectroscopy. The morphology was understood by AFM and SEM. Additionally, the study investigates the antioxidant activity and antimicrobial efficacy of so-synthesized Ag NPs against pathogenic microorganisms such as E. coli. and S. aureus. The study also assesses the anticancer activity of Ag NPs on four different cancer cell lines (A 2780, MDA-MB-231, A 375, and HepG2). The half maximal inhibitory concentration (IC50) of nano-silver sample SS-1 was about 5 μg against A 2780 and MDA-MB-231 cell lines.