Sonochemical internalization using 5-aminolevulinic acid enhances the cytotoxic effect of bleomycin

Abstract

s / Photodiagnosis and Photodynamic Therapy 12 (2015) 325–375 343 Results: The few treated cases for the moment show fading lesions in an average of 4 sessions, including an immunocompromised patient had a very quick recovery. Conclusions:Fora country likeEcuador is very important search new cheaper and efficient alternatives for the treatment of health public problems. The results do not present major problems to raise a medical guideline, however this study will be continued and should be replicated in different demographic areas throughout the country collecting statistical data over types of patients, sessions conducted and recurrences. http://dx.doi.org/10.1016/j.pdpdt.2015.07.073 Examining the effect of liposomal lapatinib in combination with PDT for the treatment of malignant gliomas Carl Fisher1,2,3,4, Carolyn Niu1,2,3,4, Warren Foltz1,2,3,4, Girgis Obaid1,2,3,4, Tayyaba Hasan1,2,3,4, Lothar Lilge1,2,3,4 1 Department of Medical Biophysics, University of Toronto, Canada 2 Department of Radiation Oncology, University Health Network, Canada 3 Wellman Center for Photomedicine, Massachusetts General Hospital, USA 4 Division of Molecular Imaging, Princess Margaret Cancer Centre, Canada Malignant gliomas are invasive and difficult to treat tumours. Of this group of tumours, Glioblastoma Multiforme (GBM) is the most common and the most aggressive. Of proposed GBM treatments, ALA-PpIX mediated Photodynamic Therapy (PDT) has been explored and researched into. The goal of our group is to examine whether combining PDT with EGFR kinase inhibitors leads to greater outcomes compared to PDT or EGFR inhibitors alone. The study proposed utilized the human cell line U87 (EGFR positive) in combination with a liposomal based EGFR inhibitor (lapatinib) that was combined with PDT treatment. Pre-PDT animals were scannedwithaBruker7T small animalMRI systemtomeasure cerebral blood flow (CBF) and tumor vascular permeability and began treatment with the liposomal lapatinib conjugation. Post-PDT animal were scanned at regular intervals to measure CBF and tumour size between the two groups. In the initial pilot study, liposomal lapatinib combined with PDT lead to significant increases in survival compared to PDT alone and demonstrated differences in both CBF and tumour response to PDT. Ongoing studies are focused on increasing the number of animals and get quantitativemeasures of CBF and cerebral blood volume both preand post-PDT. http://dx.doi.org/10.1016/j.pdpdt.2015.07.074 Sonochemical internalization using 5-aminolevulinic acid enhances the cytotoxic effect of bleomycin Tomohiro Osaki1, Yoshihiro Uto2, Masahiro Ishizuka3, Toru Tanaka3, Nobuyasu Yamanaka4, Tsukasa Kurahashi4, Kazuo Azuma1, Yusuke Murahata1, Takeshi Tsuka1, Norihiko Ito1, Tomohiro Imagawa1, Yoshiharu Okamoto1 1 Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Japan 2 Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Japan 3 SBI Pharmaceuticals Co., Ltd., Japan 4 ITO Physiotherapy & Rehabilitation, Japan Photochemical internalization (PCI) is a technology to improve the intracellular delivery ofmacromolecules andbasedon the same principle as photodynamic therapy (PDT). PCI is based on the use of photosensitizers located in endocytic vesicles that upon activation by light induce rupture of endocytic vesicles and release the macromolecules into cytosol. Bleomycin (BLM) is used for chemotherapeutic agent for PCI. The limitation of PDT and PCI is the penetration of light in the tissue. It is difficult to treat deepseated tumor with PDT and PCI. Sonodynamic therapy (SDT) is also an antitumor strategy that kills tumor cells by ultrasound and photosensitizer. Ultrasound can penetrate deep tissue and excite photosensitizer. We hypothesized that ultrasound could excite photosensitizers used for PCI, and this newmethod could improve the intracellular delivery of macromolecules. We defined this new method as sonochemical internalization (SCI). In this study, we examined whether internalization of BLM by ultrasound and 5aminolevulinic acid (5-ALA) was able to destroy tumors. In vitro and in vivo study, a significant cytotoxic effect of SCI of BLM using 5-ALA was observed. It was considered that SCI might act as an ultrasound-directed drug-delivery system by triggered release of endocytosed macromolecules into cytosol as PCI. http://dx.doi.org/10.1016/j.pdpdt.2015.07.075 Acridine orange as radiosensitizer to improve cell damage caused by radiotherapy in breast cancer cells line Cintia Teles de Andrade, Jose Dirceu Vollet-Filho, Layla Pires, Vanderlei Salvador Bagnato, Cristina Kurachi Sao Carlos Institute of Physics, University of Sao