Abstract
Recently, there is a growing demand in sustainable phytopathogens control research. Nanotechnology provides several tools such as new pesticides formulations, antibacterial nanomaterials and smart delivery systems. Metal nano-oxides and different biopolymers have been exploited in order to develop nanopesticides which can offer a targeted solution minimizing side effects on environment and human health. This work proposed a nanotechnological approach to obtain a new formulation of systemic fungicide fosetyl-Al employing ultrasonication assisted production of water dispersible nanocrystals. Moreover, chitosan was applicated as a coating agent aiming a synergistic antimicrobial effect between biopolymer and fungicide. Fosetyl-Al nanocrystals have been characterized by morphological and physical-chemical analysis. Nanotoxicological investigation was carried out on human keratinocytes cells through cells viability test and ultrastructural analysis. In vitro planktonic growth, biofilm production and agar dilution assays have been conducted on two Xylella fastidiosa subspecies. Fosetyl-Al nanocrystals resulted very stable over time and less toxic respect to conventional formulation. Finally, chitosan-based fosetyl-Al nanocrystals showed an interesting antibacterial activity against Xylella fastidiosa subsp. pauca and Xylella fastidiosa subsp. fastidiosa.
Highlights
Samples preparation follows the same protocol for the two techniques: A drop (10 μL) of each sample solution was placed on a standard carbon-coated transmission electronic microscopy (TEM) Cu-grid and let the solvent to dry at room temperature
The environmental and economic damage caused by Xylella fastidiosa subsp. pauca (Xfp) in Apulia region represented a stimulus in thTehide eenntivfiircoantimonenotfanl eawndcoemcopnooumndics danamd angeewcsayunstehdesbisymXeftphoidnsAabpluelitao creognitornol rtheperbeasecntetreiduma. sTthime urelusesairnchthoef nidewenFtiofisceattyiol-nAloffonrmewulacotimonpso, uwnhdischacnodulndewbe sfyunntchtieosnisalmaegtahinodstsXafbplpe etsot aconndtrhoulmthaen baancdteerniuvmiro.nTmheenrteaslelyarscahfeo,fisnpewartFoofsethtyisl-iAnnl ofovramtiounlaptieornssp,ewcthiviceh
Mdeomreoonvsetrra, twe ethperionvveidtrtoheaffit bcaacctyeroifaCgHro-wnannooFnoPsDon2 tmweoddiuiffmerseunptpXl.efmasetnidtieodsaosrubnsoptewciieths aCnHd-rneapnreosFeonst reduce significantly the CFU mean area. These results demonstrate the in vitro efficacy of CH-nanoFos on two different X. fastidiosa subspecies and represent the first step to understand the role of this the first step to understand the role of this nanoformulation in the treatment of X. fastidiosa
Summary
In the recent years, increased investigations have been undertaken from academy and industry research for innovative and safe solutions to control phytopathogens diffusion. The active substance for plants treatments must be proven safe for human health, animals and environment including their residues in food. The European Union (EU) regulates precisely the placing on the market and the maximum residue levels (MRLs) in food of plants protection products, including biocidal. EC regulation provides a list of potential active substances candidates, both chemical and not, which could replace products for plants protection. The analysis of these candidates must be carried out by EU countries and stakeholders. CaCO3 nanocrystals evidences have been proved the strategic role of nanopesticides to potentially control X. fastidiosa pathogen [26]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
