Sonic hedgehog regulates the position of the trigeminal ganglia

Abstract

The trigeminal ganglia differentiate in part from specialized ectodermal structures in the embryonic head termed the trigeminal placodes. However, the signals which govern the migration of trigeminal precursors and the final morphology of the ganglia are poorly defined. Here, we show that notochord or floor plate tissue can induce the formation of ectopic sensory ganglia adjacent to the developing dorsal mesencephalon. Neurons within these ganglia coexpress the transcription factors Brn3a and Islet, which together characterize primary sensory neurons throughout the developing embryo. The ectopic ganglia originate from Pax3-expressing regions of the surface ectoderm that normally contribute to the ophthalmic trigeminal (op5), and can only be induced at developmental stages during which op5 precursors are present in the mesencephalic region. The migration of trigeminal precursors is also blocked by a local source of recombinant Shh, while in mouse embryos lacking Shh, these cells continue to migrate until they fuse into a single ganglion at the ventral midline. Together, these results suggest that Shh acts to arrest the migration of sensory precursors rather than to induce sensory neurons de novo. Consistent with this hypothesis, Shh induces the expression of the proteoglycan PG-M/versican in the cranial mesoderm, which has been previously implicated in the regulation of the movement of sensory neural precursors.