Some Virulence Genes Profile of Escherichia coli Stains Isolated from Urinary Tract Infection in Iraqi patients

Abstract

Background: The urinary tract infection (UTI) is the most frequent bacterial infectious illness seen in clinical practice, accounting for considerable morbidity and high medical expenses. Escherichia coli is the most prevalent bacterium causing UTIs. The expression of a broad range of virulence factors by E. coli contributes to the severity of UTI. This research investigated the function of E. coli virulence determinants in the pathogenesis of UTI. The aim of the present study is to identify virulence genes in Uropathogenic E. coli which are responsible for UTI by isolation and identification of major microorganisms (Uropathogenic E. coli) that cause UTI and detection of antimicrobial susceptibility of the bacterial isolates. The determination of some virulence uropathagant genes such as cnf, hly, and fimH genes by PCR. Material and Methods: From March 2022 to June 2022, approximately 200 clinical urine samples were collected from patients suffering from UTI of both genders; (160) urine samples from females and (40) urine samples from males in age groups ranging from 15–50 years who attended Al-Sadr Teaching Hospital, Al-Zahrawi Surgical Hospital, and Misan Hospital for child and childbirth in Misan city, Iraq. Results: In the current study, E.coli has some virulence factors such as cnf, hly, and fimH for (40) E.coli stains were reported as a positive 4(10%), 30(75%), and and 13(32.5%) respectively. Conclusions: Our findings suggest that looking into the bacterial pathogenicity linked to UTIs might help doctors provide better care.