Abstract
Several variables which differed among lymphocyte freezing and storage protocols, including resuspending media, freezing containers, sealing methods, freezing apparatus, and storage units, were investigated. Loss of viability was variable with the freezing and thawing techniques used; however, in general, lowered viability was not dependent upon the variables tested. Good results were achieved with 3 different, inexpensive freezing devices: the modified‐Hauschka box, a mechanical freezer set at — 68° C, and a Linde BF‐5 biological freezer. Lymphocyte storage in the mechanical freezer proved satisfactory for as long as 5 months.
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