Abstract

Since the beneficial effects of B. acidophilus appear to depend upon a transformation of the intestinal flora, it follows that a mass inoculation is desirable. This means a maximum number of viable organisms per c.c. of pabulum. The usual recommendation on commercial preparations of fermented milk is that such milk be kept in a cool place preferably in the ice-box. Consequently, the influence of low temperature on the number of viable B. acidophilus in milk seemed worth investigating.The number of viable B. acidophilus in milk held in the icebox at about 97° C. was determined daily. The viable organisms were killed as follows: after 1 day—about 50 per cent.; after 2 days—about 75 per cent.; after 3 days about 90 per cent., etc. The obvious importance of having a large number of viable organisms is manifested in the necessity for increasing dosage in severe cases. In fact, it is likely that in cases reported as failures, a sufficient increase in the number of viable organisms administered might have result...

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