Some supravital observations on the “L.E.” phenomenon

Abstract

1. 1. We have reviewed the current literature on the “L.E.” cell phenomenon, the reported methods for demonstrating these cells and the opinions as to the component parts of this phenomenon. 2. 2. We have presented our method for obtaining, preparing and supravitally staining peripheral blood and bone marrow specimens, and five cases demonstrating the “L.E.” phenomenon are summarized. 3. 3. In our studied cases the “L.E.” cell has two component parts. The “L.E.” cell inclusion body has invariably been a mature, autolyzed polymorphonuclear neutrophil. The phagocytic cell, in our experience, has been shown to be either a mature polymorphonuclear neutrophilic, eosinophilic or basophilic leukocyte, with the neutrophilic leukocytes overwhelmingly predominating. 4. 4. Bone marrow examination is the most reliable means of demonstrating “L.E.” cells by our technic even though we have, on routine peripheral blood examination, found “L.E.” cells present in four of the five summarized cases. 5. 5. We present this as a simple, effective, direct method for demonstrating “L.E.” cells without resorting to special manipulative procedures of peripheral blood or bone marrow.