Abstract
Studies on the incorporation of radioactive amino acids into total protein of the anterior pituitary gland (APG) of rats were performed in order to learn more about the effects of thyroid hormones on this gland. APG incubated in Krebs-Ringer phosphate buffer (with 250 mg/100 ml glucose, without Ca++) incorporated 14C-leucine into total protein at a linear rate for about 8 hr. Omission of glucose from the incubation medium significantly decreased the rate of incorporation, while the addition of amino acids to Krebs-Ringer buffer which contained glucose had no effect. The APG from thyroidectomized (THX) rats, but not from adrenalectomized or castrated rats, had a higher rate of incorporation of radioactive amino acids than the APG of euthyroid rats. This thyroidectomyinduced incorporation could be reduced to normal within 12 hr after a single injection of either triiodothyronine (T3), thyroxine (T4) or tetraiodothyroacetic acid. Direct addition of T3 into the incubation medium also suppressed the enhanced 1...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
