Abstract
On gel filtration through Sephadex G-150, the water-soluble polysaccharide from Mahua flowers gave two homogeneous fractions. The first fraction was found to contain d-galactose, l-arabinose, l-rhamnose, d-xylose, and d-glucuronic acid in the molar ratios of ∼21:5:1:1:6. Methylation analyses were conducted on the polysaccharide and its carboxyl-reduced derivative, and the results were corroborated by those from periodate oxidation followed by Smith degradation. The anomeric configurations of the different sugar residues in the polysaccharide were determined by chromium trioxide oxidation of the acetylated polysaccharide.
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