Abstract
There is a growing interest in the encapsulation of hemoglobin (Hb) in liposomes for two reasons: (i) the resulting hemosomes have a potential use as a non-toxic, non-immunogenic red blood cell (RBC) surrogate (1–5), and (ii) they provide a useful RBC model for studying the interaction of Hb with lipid bilayers (6,7). We prepared hemosomes by dispersing various (phospho)lipids in concentrated human RBC lysate, and report here on (i) some morphological characteristics of the resulting particles; (ii) the effect of lipid composition on the amount of entrapped Hb; (iii) the kinetics of CO-binding by entrapped Hb; and (iv) the stability of the lipid membrane and of Hb in hemosomes. To shed light on the molecular mechanism of Hb-liposome interactions, the changes in the intrinsic fluorescence of Hb upon addition to small unilamellar vesicles (SUV) were also analyzed.
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