Abstract

A DPNH peroxidase has been shown in cell-free extracts of Clostridium perfringens, strain BP6K. This enzyme, together with a non-enzymic reaction between H 2O 2 and pyruvate, apparently accounts for the failure of H 2O 2 to accumulate during glucose respiration by this microorganism. Upon aging of the extracts the peroxidase activity declines. Under these circumstances an oxidase, which accumulates H 2O 2 rapidly from DPNH or HDP, can be demonstrated.

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