Abstract
During gel filtration and subsequent anion exchange chromatography the nuclease activity from cotyledons of germinating Phaseolus vulgaris L. was eluted as a single peak, containing high RNase and low inherent DNase activities. The RNase activity was unaffected by EDTA and had no particular requirement for mono- or divalent cations, but was substantially inhibited by Mn ++ and Zn ++ . Sodium dodecyl sulphate and the vanadyl ribonucleoside complex were identified as effective inhibitors of the RNase activity. The base preference for homoribonucleic acids was: poly U > poly C > poly A > poly G.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: South African journal of botany : official journal of the South African Association of Botanists = Suid-Afrikaanse tydskrif vir plantkunde : amptelike tydskrif van die Suid-Afrikaanse Genootskap van Plantkundiges
Paper Title
Journal
Date
