Abstract
SUMMARYGrapevine Bulgarian latent virus (GBLV), obtained from symptomless vines grown in Bulgaria, was readily transmitted by inoculation of sap to a restricted range of hosts. The virus was re‐inoculated into virus‐free rooted cuttings and seedlings of several grapevine cultivars without inducing symptoms. Purified preparations of GBLV contained isometric particles c. 30 nm in diameter which sedimented as three components at52, c. 120 and 1275, respectively. The slow‐sedimenting component (T) contained non‐infective protein shells, whereas the two heavier classes of particles (J5X, B2) each contained one molecule of single‐stranded ribonucleic acid (RNA) with mol. wts of 2.1 and 2.2 times 106 daltons. The RNA content of B1 and B2 components were 39 and 41 % by particle weight respectively. The capsid was composed of one type of protein with a mol. wt of 54000. GBLV did not react with antisera to any of twenty‐eight viruses with isometric particles. Its present cryptogram is R/1: 2‐2/41 +2‐1/39:S/S:S/*.
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