Abstract
Alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV) were isolated from clones of Buddleia davidii collected at Long Ashton Research Station for assessment of horticultural characteristics. The AMV isolate investigated in this study (AMV‐B) infected 18 species and cultivars of herbaceous test plants. The in vitro properties of AMV‐B were determined. The virus was purified by permeation chromatography on controlled‐pore glass beads and an antiserum prepared. AMV‐B appeared to be serologically indistinguishable from AMV isolate 15/64. The sizes and shapes of the virus particles were those of classical AMV components. The capsid protein was a single polypeptide of Mr 24 375. Buddleia seedlings inoculated with AMV‐B showed only mild chlorosis and slight distortion of leaves.
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