Abstract
A new virus, designated “crystalline-array virus” (CAV), was isolated from the grasshopper Melanoplus bivittatus (Say). Although some differences in species susceptibility were evident in infectivity assays, CAV was found to be capable of infecting and killing grasshoppers of a wide variety of species. The cytoplasm of muscle, tracheal matrix, and pericardial cells of infected grasshoppers bore numerous rod-shaped crystals consisting of spherical virus particles, approximately 13 mμ in diameter, in crystalline array. Purification of the virus was accomplished by sucrosedensity gradient ultracentrifugation. Physicochemical analyses indicated that CAV possessed a ribonucleic acid and protein content of 18–22% and 78–82%, respectively, and had a sedimentation coefficient of 42S.
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