Abstract
The carboxylesterases of bovine liver were separated electrophoretically into a rapidly migrating band and a group of four slow bands of activity. Incubation of buffered samples at room temperature resulted in a loss of activity in the rapidly migrating band and a simultaneous rearrangement of the activity in the slower bands, with the formation of three new electrophoretic variants. By molecular-sieve studies in starch gel, by two-dimensional electrophoresis, and by gel filtration in Sephadex G-200, this heterogeneous group of esterases was found to be of similar molecular size though differing in net electrical charge.
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