Abstract
Theca interna cells were collected from large porcine follicles in the follicular phase of the cycle. The theca interna layer was manually separated from the theca externa, washed thoroughly, occasionally treated with hyaluronidase solution and trypsinized. The resulting cell suspension consisted of approximately 70% theca interna cells, 8% granulosa cells, and 23% nonsteroidogenic cells. In one experiment, cells collected from the granulosa layer and the theca interna of the same follicles were compared. Specific cell types were identified by: 1) staining with Oil Red 0 (ORO) to visualize lipid droplets in the cytoplasm, 2) measuring activity of the Δ 36-hydroxysteroid dehydrogenase (Δ 36-OH SH) enzyme, and 3) quantitating estrogen production by the cultured cells.
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