Abstract
A number of plant tissues have two phosphorylase fractions (I and II) that can be separated by DEAE-cellulose chromatography. When only one is detectable, it corresponds to enzyme II. Peas differed from other legumes in showing an increase in enzyme I during seed germination. Examination of the I type enzymes, by sodium dodecyl sulphate polyacrylamide gel electrophoresis, sector cell ultracentrifugation and gel filtration, indicated that these were dimers composed of similar sub-units of MW near 90 000. When phosphorylase II enzymes were examined, the sub-unit MW was found to be higher, near 110 000 and, whereas ultracentrifugation techniques indicated a dimer of similar sub-units for the native enzyme, gel filtration gave higher MW values. Phosphorylase II from Victory Freezer peas differed from the other samples, in being able to form mixtures of dimer and tetramer.
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