Abstract
Abstract— Fluorochromation and staining possibilities of rat mast cells with basic dyes have been studied. The material consisted mainly of tongue, salivary and lymph glands, as well as decalcified material from jaws containing incisors. A very selective fluorescence for mast cells was achieved with several basic fluomchromes, but berberine sulfate, acridine orange, and thioflavine T proved especially useful. Some combinations of dyes to be used for ordinary and/or ultraviolet light are described. The most useful seemed to be acridine orangelhematoxylinleosin, acridine orangelaniline blue WS, berberine sulfatelthiazine red R. The acid dyes mainly served as background stains. Good results were achieved in ordinary light with the basic dye methyl green, which also revealed interesting color change phenomena (“allochromasia”).
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