Abstract
1. 1. Pipa carvalhoi. an anuran from northeast Brazil that lives in an aquatic environment even as an adult, has four hemoglobin components which have been identified by starch-gel electrophoresis and separated by CM-cellulose chromatography. 2. 2. Some structural and functional properties of its hemoglobins were determined and compared with those of terrestrial anurans. The oxygen affinity, Bohr effect and rate of methemoglobin formation in the presence of alkali, urea and sodium benzoate have features similar to those of tadpole hemoglobins already described. 3. 3. The number of reactive thiol groups are 1.5–2 per tetramer of hemoglobin, in contrast to the hemoglobins of tadpoles, which lack thiol groups.
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