Abstract
Relative surface charge, hydrophobicity of bacterial cells and their affinity to substrate during copper bioleaching from post-flotation wastes was studied. Surfaces of three strains showed negative relative surface charge and the other ones were close to neutral. All strains showed hydrophilic surfaces. Extracellular polymers produced by bacteria were isolated and their influence on adhesion to different surfaces was examined. Exopolymer production was generally the lowest in full organic medium and the highest on poor mineral medium and it was negatively correlated with copper solubilisation. Produced exopolymer was highly resistant to acid hydrolysis and contained hydrocarbons, saturated and unsaturated fatty acids, aromatic acids and aromatic acid esters as was shown by GC- MS analysis. The effects of bacterial exopolymer produced in bioleaching process on substrate surface was investigated. The surface tension of water, culture liquid and exopolymer solution and their work of adhesion to hydrophobic and hydrophilic substrate was examined.
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