SOME FACTORS AFFECTING MICROPROPAGATION OF Haplophyllum tuberculatum L

Abstract

This study was carried out at Plant Tissue Culture Laboratory, Faculty of EnvironmentalAgricultural Sciences, El-Arish University, North Sinai Governorate, Egypt during 2014 to 2017.Effect of three medium types, Murashige and Skoog (MS), Linsmaier and Skoog (LS) and Lloyd andMcCown (WPM), two explant types (shoot-tip and single node cutting), three cytokinin types(Benzyladenine, Kinetin and 2-isopenteny1 adenine), two additives; viz., s (thiamine and pyridoxinehydrochloride) and amino acids (asparagine and arginine) and two gelling agents (guar seed powderand locust bean seed powder) combined with agar on micropropagation of Haplophyllum tuberculatumwere investigated. For rooting, the effect of three auxins (Indole acetic acid, Indol butyric acid andNaphthalene acetic acid) were studied in combination with three medium strengths (full, half andquarter) combined with the best mixture (0.50 mgl-1 Kin + 6 gl-1 agar with 2 gl-1guar seed powder) ofgelling agents. Obtained results showed that Murashige and Skoog (MS) was the best medium withsingle node explant as starting material during establishment stage. Multiple shoots were obtained onMS medium supplemented with 1.0 mgl-1 Kinetin (Kin) combined with 0.1 mgl-1 α-naphthalene aceticacid (NAA). Thiamine and arginine were the best vitamins and amino acid at 0.1 mgl-1 and 0.50 mgl-1,respectively. Agar at 6 gl-1 + guar seed powder at 2 gl-1 was the best gelling agent treatment. Halfstrength MS medium with 0.50 mgl-1 Kin + 0.5 mgl-1 NAA and 6 gml-1 agar with 2 gml-1 guar seedpowder was the best combination for rooting were successfully (85% survival) acclimatized in mixtureof peatmoss, vermiculite, washed sterilized sand and perlite (1:1:1:1, V/V/V/V).