Abstract
Diluted semen from the bull, boar, and turkey was plunged into liquid nitrogen. After separation of the cells from the extracellular medium, the total releasable amounts of glutamic oxaloacetic transaminase (GOT), lactic dehydrogenase (LDH), cholinesterase, acid phosphatase, and alkaline phosphatase were determined. Turkey semen was low in enzyme content when compared to the bull and boar. Correlations between sperm cell concentration and enzyme concentration along with the amounts of enzyme in the extracellular medium after minimum and maximum damage indicated that GOT was present primarily in the cell. Therefore, of the five enzymes studied, GOT release into the extracellular medium was the best indicator of cell damage. Comparison of filtering semen through cellulose powder with discontinuous centrifugation to separate the cells from the extracellular medium was studied. Discontinuous gradient centrifugation resulted in significantly lower release of GOT from the cells in the boar and turkey than filtration. Thus discontinuous gradient centrifugation was the superior method for separating spermatozoa from the extracellular medium.
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