Abstract

A biochemical study was conducted to assess the relative presence of some enzymes of gluconeogenesis in various fractions—the cyst wall, cyst fluid and zoites—of sarcocysts of Sarcocystis fusiformis obtained from the oesophageal muscles of naturally infected Indian water buffalo ( Bubalus bubalis). The activities of fructose 1,6-diphosphatase and malic enzyme were beyond detectable limits. Phosphoenol pyruvate carboxykinase (PEPCK) activity was maximally present in the zoites, whereas glucose 6-phosphates activity was highest in the cyst wall. PEPCK seemed to play a crucial role in carbon dioxide fixation metabolism.

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